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Keywords: Centropomus poeyi snook larviculture gene expression RT-qPCR digestive enzyme
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poeyi larvae showed early and high digestive enzyme expressions, especially TRY, which indicate that could be possible to start the weaning process at 25 DAH, which could be shorter compared than other Centropomid species. Finally, CNP gene expression had presented three peaks at 2 DAH (yolk-sac absorption), 19 and 23 DAH when larvae were fed with Artemia nauplii. AMY gene expression was low detected from hatching onwards and showed a slight peak at 10 DAH when larvae were fed with Artemia nauplii. TRY gene expression showed the maximum peak at 17 DAH meanwhile, BAL gene expression showed variations during larviculture, however, three peaks were observed at 10, 17 and 25 DAH, which appears to correspond to changes in live food supply (rotifers and Artemia nauplii). In this study, the gene expression of trypsin, (TRY), bile-salt dependent lipase (BAL), amylase (AMY), and cytosolic non-specific dipeptidase (CNP) was investigated during the larval period from embryo (0 days after hatching, DAH), eleuteroembryo (1 DAH) until 25 DAH using RTq-PCR approach and was normalized with the elongation factor 1-( gene (EF1-α). However, the basic biological knowledge of the digestive physiology is still unknown. For this reason, some recent studies related to its culture have been done.
CFX MANAGER 3.1 SOFTWARE
CFX Manager software quick guides for system installation, protocol, plate, data.Mexican snook (Centropomus poeyi) is one of the most important commercial species in Southeast Mexico, which has been overexploited by fisheries. Please note, that the Bio-Rad CFX Connect™ real-time thermal cycler is a licensed real-time.
CFX MANAGER 3.1 SERIES
click on Replicate Series to tell the software, that you have technical triplicates loaded horizontally: Replicate size =3. CFX Manager software quick guides for system installation, protocol, plate, data.
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CFX MANAGER 3.1 MANUAL
Select Settings For information about safety labels used in this manual and on the CFX96. CFX Manager software automatically subtracts the baseline from well data. Remove all mode, click and drag the threshold line to manually position the line. is licensed by Life Technologies Corporation to sell reagents containing SYBR® CFX Manager software video tutorial.
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Use CFX Manager software to set up experiments and analyze data with the PCR Detection Systems, includes installation CD, quick guides, and manual. CFX Manager software quick guides for system installation, protocol, plate, data analysis Version 3.1, experiment setup and data analysis software for all CFX Real-Time PCR Detection Systems, includes installation CD, quick guides, and manual. Prohibited without written permission of Bio-Rad Laboratories, Inc. BIO RAD CFX MANAGER SOFTWARE MANUAL > DOWNLOADīIO RAD CFX MANAGER SOFTWARE MANUAL > READ ONLINEĬfx manual bio rad qpcr standard curve cfx maestro technical replicatesbio rad youtube bio rad cfx manager gene study bio rad cfx96 plate setup biorad qpcr bio rad melting curve analysis